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) (a) Schematic of Nipkow disc FLIM microscope with (b) optically sectioned FLIM image of live cells expressing EGFP (left) and EGFP-mRFP FRET construct with (c) corresponding lifetime histogram and (d) FLIM-FRET image recorded with 5 s acquisition of MDCK cells expressing EGFP-Raf-RBD and H-Ras-mRFP, stimulated with EGF for 10 min.

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